Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2 , Rabbit , Novus NBP2-22218 , 1:200 , Overnight, RT.

Techniques: Recombinant, Software

Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2 , Rabbit , Novus NBP2-22218 , 1:200 , Overnight, RT.

Techniques:

Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2, Rabbit , Novus , Cat# NBP2-22218; RRID: AB_2335858.

Techniques: Recombinant, Software

Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2, Rabbit , Novus , Cat# NBP2-22218; RRID: AB_2335858.

Techniques:

Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2 , Rabbit , Novus NBP2-22218 , 1:200 , Overnight, RT.

Techniques: Recombinant, Software

Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT2 , Rabbit , Novus NBP2-22218 , 1:200 , Overnight, RT.

Techniques:

Journal: Cells

Article Title: Pseudoislet Aggregation of Pancreatic β-Cells Improves Glucose Stimulated Insulin Secretion by Altering Glucose Metabolism and Increasing ATP Production

doi: 10.3390/cells11152330

Figure Lengend Snippet: Pseudoislet formation alters expression and activity of glycolytic and TCA cycle enzymes. MIN6 cells (passage 26–29) were cultured in either standard cell culture plates (ML) or in un-coated Petri-dishes (PI) for 5 days. ( A , B ) Changes in enzyme activity for glycolytic enzymes ( A ) or TCA cycle enzymes ( B ) was determined on cell lysates from ML and PI and activity corrected for protein content. ( C , D ). Changes in protein expression of HK ( C ) and GLUT2 ( D ) were determined using Western blotting and expressed relative to β-actin. Results are expressed as fold change relative to ML ( A , B ) or as GLUT2 or HK: β-actin ratio ( C , D ). n = 3–8. * p < 0.05, ** p < 0.01, # p < 0.05 using an unpaired t -test.

Article Snippet: Membranes were probed with primary antibody (PIM: HP2-1000Kit, Hypoxyprobe (Burlington, MA, USA); GLUT2: NBP2-22218, Novus Biologicals (Cambridge, UK); HK I: SC-6517, Santa Cruz (Heidelberg, Germany); β-actin: 66009-1-Ig, Proteintech (Manchester, UK)) at 4 °C overnight.

Techniques: Expressing, Activity Assay, Cell Culture, Western Blot

Journal: Cell Reports Medicine

Article Title: Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing

doi: 10.1016/j.xcrm.2020.100125

Figure Lengend Snippet: URI Controls PDX1 Expression and β Cell Identity in Mice (A) WB of 7-week-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases, normalized to GLUT2 expression. (B) qRT-PCR of indicated mRNA from 7-week-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases, normalized to β-actin mRNA expression (n = 6 and 5). (C) IF of insulin and PXD1 in 2-day-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases (post-natal). (D–F) Quantification of PDX1 nuclear expression (D), percentage of PDX1 + cells (E), and insulin expression (F) in 2-day-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases (n = 6 and 7). (G) Pancreas to body weight of 2-day-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre mice (n = 5). (H) Area of pancreatic islets per field in 2-day-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases (n = 6 and 7). (I) Fasting glucose levels of 2-day-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre mice (n = 7 and 8). (J) Fasting glucagon levels of 7-week-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre mice (n = 12 and 11). (K) Confocal IF of insulin (red) and glucagon (green) in 7-week-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases. (L) Quantification of polyhormonal cells per field in 7-week-old Pdx1-cre and Uri1 flox/flox ; Pdx1-cre pancreases. Up to 5 fields in 1 cut per sample were examined (n = 5 and 6). (M) Confocal IF of insulin and glucagon from CVB4-infected human pancreas xenografts in mice. Asterisks represent bihormonal cells. (N) Quantification of bihormonal—insulin and glucagon—positive cells. Up to 5 fields in 1 cut per sample were examined (n = 10 and 7). Data are means ± SEMs. Statistical analysis done using Student’s t test. ∗p < 0.05; ∗∗p < 0.01. Scale bar, 25 μm in (C) and 50 μm in (K) and (M).

Article Snippet: Rabbit polyclonal anti-Glut2 , Novus Biologicals , NBP2-22218; RRID: AB_2335858.

Techniques: Expressing, Quantitative RT-PCR, Infection

Journal: Cell Reports Medicine

Article Title: Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing

doi: 10.1016/j.xcrm.2020.100125

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Glut2 , Novus Biologicals , NBP2-22218; RRID: AB_2335858.

Techniques: Blocking Assay, Recombinant, Electrophoresis, Bradford Protein Assay, Protease Inhibitor, Electron Microscopy, Avidin-Biotin Assay, Plasmid Preparation, Activity Assay, Fluorescence, Transfection, In Situ, Labeling, Gel Extraction, Purification, Enzyme-linked Immunosorbent Assay, Sequencing, Software

Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: Expression of ChREBP, GLUT2 and GLUT1 in different clinical stages

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Expressing

Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: Histograms of protein expression in different liver tissues. Histograms of scores of ChREBP ( a ), GLUT2 ( b ), GLUT1( c ) expression levels in different stages of liver cancer. 0, absent of positive signal; 1, mild staining; 2, middle staining; 3, strong staining; 4, very strong staining. One-way ANOVA was used to analyze the correlation between protein expression and liver malignancy. Student-T test or Mann-Whitney U test was used to analyze the protein expression differences between 2 different clinical stages. **, P < 0.01; ***, P < 0.001

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Expressing, Staining, MANN-WHITNEY

Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: GLUT1 but not GLUT2 protein co-expressed in ChREBP-positive hepatocytes in HCC . Immunohistochemistry of ChREBP-positive malignant hepatocytes ( a ) showing GLUT2 negative ( c ) but GLUT1 positive ( e ) staining. Arrow heads indicating the same cell. Immunohistochemistry of ChREBP-negative malignant hepatocytes ( b ) showing GLUT2 positive on the membrane ( d ) but GLUT1 negative staining ( f ). Arrow indicating the same cell. Scale bar = 30 μm

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Immunohistochemistry, Staining, Membrane, Negative Staining